Biotechnology: Principles and Processes MCQ Questions for Class 12 Biology Chapter 11 with Answers

Find here the NCERT chapter-wise Multiple Choice Questions from Class 12 Biology book Chapter 11 Biotechnology: Principles and Processes with Answers Pdf free download. This may assist you to understand and check your knowledge about the chapters. Students also can take a free test of the Multiple Choice Questions of Biotechnology: Principles and Processes. Each question has four options followed by the right answer. These MCQ Questions are selected supported by the newest exam pattern as announced by CBSE.


NCERT MCQ Chapters for Class 12 Biology

Q1. Which of the following is not used to transfer the recombinant DNA into the host ?

(i) Micro-injection method
(ii) Gene gun method
(iii) Bioreactors
(iv) Disarmed pathogen vectors

(iii) Bioreactors


Q2. Which one is called molecular scissors?

(i) Endocrine
(ii) Ribonuclease
(iii) Restriction enzymes
(iv) Exonuclease

(iii) Restriction enzymes


Q3. Restriction’ in Restriction enzyme refers to:

(i) Cleaving of phosphodiester bond in DNA by the enzyme
(ii) Cutting of DNA at specific position only
(iii) Prevention of the multiplication of bacteriophage in bacteria
(iv) All of the above.

(iv) All of the above.


Q4. Two microbes found tb be very useful in Genetic Engineering are:

(i) Excherichia coli and Agrobacterium tumefaciens
(ii) Vibrio cholarae and a tailed bacteriophage
(iii) Diplococcus sp. and Pseudomonas sp.
(iv) Crown gall bacterium and Caenorhabdities elegans

(i) Excherichia coli and Agrobacterium tumefaciens


Q5. Which of the following is required to perform polymerase chain reaction ?

(i) Primers, dNTPs and DNA polymerase
(ii) DNA, CaCl2 and nuclease
(iii) Mg + 2, DNA
(iv) Both (i) and (iii)

(iv) Both (i) and (iii)


Q6. Brief exposure of cells to an electric field also allow the bacteria to take up DNA and this process is called as

(i) Apheresis
(ii) Electroporation
(iii) Electrolysis
(iv) Electrophoresis

(ii) Electroporation


Q7. Who discovered recombinant DNA (rDNA) technology?

(i) James D. Watson
(ii) Walter Sutton and Avery
(iii) Stanley Cohen and Herbert Boyer
(iv) Har Gobind Khorana

(iii) Stanley Cohen and Herbert Boyer


Q8. Which of the given statement is correct in the context of observing DNA separated by agarose gel electrophoresis?

(i) DNA can be seen in visible light
(ii) DNA can be seen without staining in visible light
(iii) Ethidium bromide stained DNA can be seen in visible light
(iv) Ethidium bromide stained DNA can be seen under exposure to UV light.

(iv) Ethidium bromide stained DNA can be seen under exposure to UV light.e


Q9. DNA obtained for DNA finger printing:

(i) W.B.C.
(ii) Hair root cells
(iii) Body fluids
(iv) None of these

(i) W.B.C.


Q10. Which one of the following is not a correct match ?

(i) Tumour inducing – Ti plasmid
(ii) DNA probe – Identifies the desired DNA
(iii) PCR – DNA staining
(iv) Agarose – Seaweeds

(iii) PCR – DNA staining


Q11. Electroporation facilitates introduction of foreign DNA into the target organism by

(i) Changing the porosity of the cell wall
(ii) Changing the electric potential of the cell wall
(iii) Lysis of the cell wall
(iv) Active transport across the cell wall

(i) Changing the porosity of the cell wall


Q12. The transfer of genetic material from one bacterium to another through the mediation of a vector-like virus is termed as:

(i) Transduction
(ii) Conjugation
(iii) Transformation
(iv) Translation.

(i) Transduction


Q13. Who discovered Restricted endonucleas enzymes?

(i) Smith and Nathane
(ii) Baiger
(iii) Waxman
(iv) Fleming

(i) Smith and Nathane


Q14. Enzyme ‘Taq polymerase’ used in PCR, has been isolated from bacterium

(i) Agrobacterium tumefaciens
(ii) Thermus aquaticus
(iii) Streptomyces albus
(iv) Escherichia coli

(ii) Thermus aquaticus


Q15. The extraction of DNA from the agrose gel is called as

(i) Elution
(ii) Ligation
(iii) Transformation
(iv) Isolation

(i) Elution


Q16. Helium is used in Biolistic gun as

(i) It is a light gas
(ii) It acts as a propellant
(iii) It is an inert gas
(iv) Tungsten coated with DNA can be easily passed in the presence of Helium

(ii) It acts as a propellant


Q17. Rising of dough is due to

(i) Multiplication of yeast
(ii) Production of CO2
(iii) Emulsification
(iv) Hydrolysis of wheat flour starch into sugars.

(ii) Production of CO2


Q18. Transgenic plants are develop by:

(i) Introducing of foreign fenes
(ii) Clone and genetically modified genes
(iii) Genetic engineering
(iv) Purified genes

(i) Introducing of foreign fenes


Q19. Process used for amplification or multiplication of DNA in DNA fingerprinting is

(i) polymerase chain reaction
(ii) southern blotting
(iii) northern blotting
(iv) none of these.

(i) polymerase chain reaction


Q20. Restriction endonucleases are enzymes which

(i) make cuts at specific positions within the DNA molecule
(ii) recognize a specific nucleotide sequence for binding of DNA ligase
(iii) restrict the action of the enzyme DNA polymerase
(iv) remove nucleotides from the ends of the DNA molecule

(i) make cuts at specific positions within the DNA molecule


Q21. A recombinant DNA molecule can be produced in the absence of the following:

(i) Restriction endonuclease
(ii) DNA ligase
(iii) DNA fragments
(iv) E. coli.

(iv) E. coli.


Q22. Plasmid is a:

(i) Fungus
(ii) Plasmid
(iii) Part of Plasma membrane
(iv) Extra chromosomal DNA in bacterial cell

(iv) Extra chromosomal DNA in bacterial cell


Q23. Which of the following is not used to transfer the recombinant DNA into the host ?

(i) Micro-injection method
(ii) Gene gun method
(iii) Bioreactors
(iv) Disarmed pathogen vectors

(iii) Bioreactors


Q24. Which one of the following is a case of wrong matching?

(i) Vector DNA – Site for t-RNA synthesis
(ii) Somatic hybridisation – Fusion of two diverse cells
(iii) Callus – Unorganised mass of cell produced in tissue culture
(iv) Micropropagation – In vitro production of plants in large numbers

(i) Vector DNA – Site for t-RNA synthesis


Q25. In agarose gel electrophoresis, DNA molecules are separated on the basis of their:

(i) Charge only
(ii) Size only
(iii) Charge to size ratio
(iv) All of the above.

(ii) Size only


Q26. Restriction endonuclease cuts:

(i) One stand of DNA at specific site
(ii) Both strands of DNA
(iii) Both strands of DNA at any site
(iv) Single strand of RNA

(ii) Both strands of DNA


Q27. A device in which large volume of living cells are cultured in order to get a specific product is called

(i) PCR
(ii) agitator
(iii) bioreactor
(iv) assimilator.

(iii) bioreactor


Q28. For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of:

(i) Silver or Platinum
(ii) Gold or Tungsten
(iii) Silicon or Platinum
(iv) Platinum or Zinc

(ii) Gold or Tungsten


Q29. Golden rice is a promising transgenic crop. When released for cultivation, it will help in :

(i) High lysin content
(ii) Pest resistance
(iii) High protien content
(iv) High vitamin A content

(iv) High vitamin A content


Q30. An enzyme catalysing the removal of nucleotides from the ends of DNA is

(i) endonuclease
(ii) exonuclease
(iii) DNA ligase
(iv) Hind II.

(ii) exonuclease


Q31. Restriction enzymes belongs to a larger class of enzymes called?

(i) Nucleases
(ii) Glcucatase
(iii) Chitinase
(iv) Protease

(i) Nucleases


Q32. While isolating DNA from bacteria, which of the following enzymes is not used?

(i) Lyozyme
(ii) Ribonuclease
(iii) Deoxyribonuclease
(iv) Protease.

(iii) Deoxyribonuclease


Q33. Triticale, first man made cereal crop, has been obtained by crossing wheat with:

(i) Rye
(ii) Pearl millet
(iii) Sugarcane
(iv) Barley

(iv) Barley


Q34. Who is the father of genetic engineering ?

(i) Steward Linn
(ii) Stanley Cohen
(iii) Paul Berg
(iv) Kary Mullis

(iii) Paul Berg


Q35. Insertional inactivation helps in

(i) X-gal breakdown
(ii) Identification of recombinant clones
(iii) Ampicillin resistance
(iv) Functioning of B-galactosidase

(ii) Identification of recombinant clones


Q36. An antibiotic resistance gene in a vector usually helps in the selection of:

(i) Competent cells
(ii) Transformed cells
(iii) Recombinant cells
(iv) None of the above.

(ii) Transformed cells


Q37. DNA element with ability to change its position is called:

(i) Cistron
(ii) Transposon
(iii) Intron
(iv) Ricon

(iii) Intron


Q38. The term ’chemical knife’ refers to

(i) polymerases
(ii) endonucleases
(iii) ribonucleases
(iv) cellulases.

(ii) endonucleases


Q39. Who among the following was awarded the Nobel Prize for the development of PCR technique?

(i) Herbert Boyer
(ii) Hargobind Khorana
(iii) KaryMullis
(iv) Arthur Kornberg.

(iii) KaryMullis


Q40. The term ’recombinant DNA’ refers to

(i) DNA of the host cell
(ii) DNA with a piece of foreign DNA
(iii) DNA with selectable marker
(iv) DNA with more than one recognition sites.

(ii) DNA with a piece of foreign DNA


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